ELISpot Flex: Rat IFN-γ (HRP)

ELISpot Flex: Rat IFN-γ (HRP)

3221-2H

Publications: 10
Documents
Questions?
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMTAM11
Detection mAbMTAM8, biotin
Enzyme conjugateStreptavidin-HRP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 2

ELISpot substrate: TMB for HRP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting rat IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Rat IFN-γ (HRP)
ApplicationELISpot
AnalyteIFN-γ
ReactivityRat
Specificity

This kit is based on a matched pair of mAbs specific for rat IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
Rat spleen cells (100,000 cells/well) were incubated overnight in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/Protocol
SDS

Featured product, Published August 17, 2022

Replacement of rat IFN-γ antibodies
We have updated our rat IFN-γ antibodies, ELISA, ELISpot, and FluoroSpot kits.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
Loading publications...

IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFNG2, If2f
Cell typeT cell, Tc, Th1, NK cell
Gene ID25712
1 / 1

ELISpot Plus: Rat IFN-γ (HRP)